| Gene name |
SPAC57A10.01 |
| Gene ID |
19/D07 |
| Gene synonyms/obsolete |
pas1;
SPAC19E9.03 |
| Gene product |
Pcl-like cyclin;
involved in start control point of mitotic cell cycle;
activator of the Res2p-Cdc10p transcriptional factor
complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1221 |
| ORF length (spliced) |
|
| Entry clone length |
1221 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
98T:C / 818T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC57A10.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGAGCTTTGCTCGAGTC |
| Rev primer name |
SPAC57A10.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGTAACTGCGACGCTTA |
| Amino acid length |
411 |
| Molecular weight |
45.3 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |