Gene name |
SPAC1D4.05c |
Gene ID |
19/C12 |
Gene synonyms/obsolete |
|
Gene product |
ER lumen protein
retaining receptor activity; involved in protein-ER retention
|
Entry clone |
Cloned |
ORF length (unspliced) |
1216 |
ORF length (spliced) |
1164 |
Entry clone length |
1216 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
446T:C / 972T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1D4.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTGGAAGTCGTGGA |
Rev primer name |
SPAC1D4.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAGGAGATAGCATCTAAA |
Amino acid length |
387 |
Molecular weight |
45.4 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPFRIGLLVI/LEILRRFLWL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |