| Gene name |
SPBC1709.14 |
| Gene ID |
19/C09 |
| Gene synonyms/obsolete |
|
| Gene product |
peptide N-glycanase;
involved in deglycosylation; involved in proteasome-dependent
pathway |
| Entry clone |
Cloned in 2004
trial_also cloned#/ 3' FS at 1st |
| ORF length (unspliced) |
1213 |
| ORF length (spliced) |
1002 |
| Entry clone length |
1213 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1709.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTTCATGCGATTTC |
| Rev primer name |
SPBC1709.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTCCTGCTTCCCCTCTT |
| Amino acid length |
333 |
| Molecular weight |
39.2 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol;
nuclear envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol; nuclear envelope |
| Microscope used for
observation |
DeltaVision |