Gene name |
SPBC119.12 |
Gene ID |
19/C05 |
Gene synonyms/obsolete |
|
Gene product |
Golgi matrix protein
predicted; involved in intracellular protein transport;
involved in Golgi organization and biogenesis; similar to
S. cerevisiae YOR216C |
Entry clone |
Cloned |
ORF length (unspliced) |
1206 |
ORF length (spliced) |
|
Entry clone length |
1206 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC119.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGAAAATGCAGGAAA |
Rev primer name |
SPBC119.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAGTCATGGAAAGAACGT |
Amino acid length |
401 |
Molecular weight |
46.1 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQEDLSRLNL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|