| Gene name |
SPAC25B8.13c |
| Gene ID |
19/A07 |
| Gene synonyms/obsolete |
isp7 |
| Gene product |
2 OG-Fe(II) oxygenase
superfamily; involved in sexual differentiation; no apparent
Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1194 |
| ORF length (spliced) |
|
| Entry clone length |
1194 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
25T:C / 328G:A /
340G:T / 885T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC25B8.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTCGCAACTAATTGA |
| Rev primer name |
SPAC25B8.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCAACTTCCAATGAGGTG |
| Amino acid length |
397 |
| Molecular weight |
43.8 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |