| Gene name |
SPBC577.15c |
| Gene ID |
19/A03 |
| Gene synonyms/obsolete |
|
| Gene product |
TPR repeat protein;
histone binding; NASP domain; involved in establishment and/or
maintenance of chromatin architecture; predicted coiled-coil
region; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1191 |
| ORF length (spliced) |
|
| Entry clone length |
1191 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC577.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCTGATACGAAAAC |
| Rev primer name |
SPBC577.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCTTCTTTTTCTTATCT |
| Amino acid length |
396 |
| Molecular weight |
43.9 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
372 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|