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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC25A8.02
Gene ID 18/E11
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1173
ORF length (spliced)
Entry clone length 1173
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC25A8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTGCATGTCAATAAATG
Rev primer name SPAC25A8.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGAAATAAGCTGATATGGT
Amino acid length 390
Molecular weight 45.3
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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