| Gene name |
SPAC513.06c |
| Gene ID |
17/D07 |
| Gene synonyms/obsolete |
|
| Gene product |
dimeric dihydrodiol
dehydrogenase; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1107 |
| ORF length (spliced) |
|
| Entry clone length |
1107 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
69G:A / 656A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC513.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTCTATGAGTGGTGC |
| Rev primer name |
SPAC513.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGCAGTGGTATAACGCAAA |
| Amino acid length |
368 |
| Molecular weight |
41.5 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|