Gene name |
SPCC622.17 |
Gene ID |
17/C08 |
Gene synonyms/obsolete |
apn1 |
Gene product |
AP endonuclease; DNA
(apurinic or apyrimidinic site) lyase, endonuclease;
endonuclease IV family |
Entry clone |
Cloned |
ORF length (unspliced) |
1101 |
ORF length (spliced) |
|
Entry clone length |
1101 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
699T:C / 1087A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC622.17.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGTGCAATAAACAAAGC |
Rev primer name |
SPCC622.17.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCATCCAAGCTCTTAACT |
Amino acid length |
366 |
Molecular weight |
41.5 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|