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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC582.09
Gene ID 17/C06
Gene synonyms/obsolete
Gene product involved in peroxisome biogenesis; involved in medium-chain fatty acid beta-oxidation
Entry clone Cloned
ORF length (unspliced) 1101
ORF length (spliced) 717
Entry clone length 1101
No. of intron 3
Sequence status Finished
Sequence results 548A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC582.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGTGATCTTTGAAAA
Rev primer name SPBC582.09.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAACTACCAATCCATGTT
Amino acid length 238
Molecular weight 26.4
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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