| Gene name |
SPAC186.03 |
| Gene ID |
16/H02 |
| Gene synonyms/obsolete |
|
| Gene product |
L-asparaginase;
similar to Sp SPAC977.12 and SPBPB8B6.05c and SPBPB21E7.09
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1083 |
| ORF length (spliced) |
|
| Entry clone length |
1083 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
390T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC186.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGGAGATCAATAATCAG |
| Rev primer name |
SPAC186.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATACTCAAGGCTGAATATA |
| Amino acid length |
360 |
| Molecular weight |
38.7 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ambiguous structure;
Golgi? |
| Comments for localization |
ER-Golgi? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |