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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC736.15
Gene ID 16/B07
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1056
ORF length (spliced)
Entry clone length 1056
No. of intron 0
Sequence status Finished
Sequence results 243T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC736.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGAACCGTGCGTACTC
Rev primer name SPCC736.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAACCTGGACGGCGGTT
Amino acid length 351
Molecular weight 39.8
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) clear filamentous structure; short filamentous structure
Comments for localization microtubules?; short filamentous structure near periphery
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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