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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC14C4.10c
Gene ID 16/A10
Gene synonyms/obsolete
Gene product Nudix family hydrolase; involved in the hydrolysis of nucleoside diphosphate derivatives; no apparent Sc ortholog
Entry clone Cloned in 2006 trial
ORF length (unspliced) 1052
ORF length (spliced) 990
Entry clone length 1052
No. of intron 1
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC14C4.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGTTGGAATTGGACTT
Rev primer name SPAC14C4.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAGATTTGTAGTAGGCTGAA
Amino acid length 329
Molecular weight 38.2
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LELDLLTGLQL/LPYMPSLRL/LLVGFLFRLFL
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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