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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC553.10
Gene ID 16/A08
Gene synonyms/obsolete
Gene product Sp specific families; serine-rich protein; hypothetical protein; GPI anchored protein (pers. comm. Birgit Eisenhaber); glycoprotein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1050
ORF length (spliced)
Entry clone length 1050
No. of intron 0
Sequence status Finished
Sequence results 400T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC553.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTGTTCAAAATTTCCTT
Rev primer name SPCC553.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAGTGAGAGAGAGAGCG
Amino acid length 349
Molecular weight 35.9
Isoelectric point (calc.) 7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFKISFLAL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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