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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC27D7.06
Gene ID 15/D08
Gene synonyms/obsolete
Gene product electron transfer flavoprotein (alpha subunit)
Entry clone Cloned
ORF length (unspliced) 1026
ORF length (spliced)
Entry clone length 1026
No. of intron 0
Sequence status Finished
Sequence results 531T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC27D7.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTAAGGACTACTAGCCG
Rev primer name SPAC27D7.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTTTTTCAGTAAGCTCC
Amino acid length 341
Molecular weight 36.3
Isoelectric point (calc.) 7.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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