Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.12
Gene ID 15/C12
Gene synonyms/obsolete yakC; SPAC21E11.01
Gene product aldo/keto reductase; AKR13 family; ADPH hydrogen donor; pyridoxal IS NOT substrate; reduces 2-nitrobenzaldehyde
Entry clone Cloned
ORF length (unspliced) 1023
ORF length (spliced)
Entry clone length 1023
No. of intron 0
Sequence status Finished
Sequence results 501C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTATCCCTACTCGTAA
Rev primer name SPAC1F7.12.Rv
Rev primer SEQ AGAAAGCTGGGTACTTGGGCATAGGAGGGGTA
Amino acid length 340
Molecular weight 37.7
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDLSLKRLGI
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.