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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC4C3.03
Gene ID 15/B06
Gene synonyms/obsolete
Gene product homoserine kinase
Entry clone Cloned
ORF length (unspliced) 1017
ORF length (spliced)
Entry clone length 1017
No. of intron 0
Sequence status Finished
Sequence results 977T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC4C3.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGAAATTTCAAATAAA
Rev primer name SPBC4C3.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGTATTTGACTGTTGCA
Amino acid length 338
Molecular weight 36.6
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LANEIAKLGL/LQMMDYVLMI
Localization (YFP) nucleus>=cytosol
Comments for localization cytoplasmic dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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