Gene name |
SPAC1071.03c |
Gene ID |
15/B04 |
Gene synonyms/obsolete |
|
Gene product |
sequence orphan;
hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1017 |
ORF length (spliced) |
|
Entry clone length |
1017 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
85T:C / 421G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1071.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTTCGATTCTGGGAAA |
Rev primer name |
SPAC1071.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATATTGTATTTTTTAGGC |
Amino acid length |
338 |
Molecular weight |
38.7 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTFSLSNLFL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |