| Gene name |
SPBC1734.13 |
| Gene ID |
14/C07 |
| Gene synonyms/obsolete |
atp3 |
| Gene product |
mitochondrial
F1-ATPase (gamma subunit); ATP synthesis coupled proton
transport |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
985 |
| ORF length (spliced) |
906 |
| Entry clone length |
985 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1734.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTCGTCAAACGCTAAC |
| Rev primer name |
SPBC1734.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCCAAAGAGTTTGCACCA |
| Amino acid length |
301 |
| Molecular weight |
33.5 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |