| Gene name |
SPAC732.01 |
| Gene ID |
13/D06 |
| Gene synonyms/obsolete |
vma11 |
| Gene product |
ATP synthase subunit;
V-type ATPase subunit; vacuolar proton pump component; V0
sector; proteolipid subunit |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
952 |
| ORF length (spliced) |
489 |
| Entry clone length |
952 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC732.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAGTAATTTATGGTA |
| Rev primer name |
SPAC732.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAACAAACGTTTGAAGTC |
| Amino acid length |
162 |
| Molecular weight |
16.7 |
| Isoelectric point (calc.) |
7.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
4 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LILIFAEVLGL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |