| Gene name |
SPAC9.12c |
| Gene ID |
12/H06 |
| Gene synonyms/obsolete |
atp12 |
| Gene product |
F1 ATPase chaperone;
ATP synthesis coupled proton transport; involved in
mitochondrial F1-F0 ATPase assembly |
| Entry clone |
Cloned |
| ORF length (unspliced) |
925 |
| ORF length (spliced) |
864 |
| Entry clone length |
925 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
131A:G / 185A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC9.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTCGATCTTTACAATT |
| Rev primer name |
SPAC9.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTGGGCATGTTCATTACTT |
| Amino acid length |
287 |
| Molecular weight |
33.1 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |