| Gene name |
SPAC1851.02 |
| Gene ID |
12/D10 |
| Gene synonyms/obsolete |
|
| Gene product |
fatty acyltransferase;
1-acylglycerol-3-phosphate O-acyltransferase activity;
involved in sphingolipid metabolism; involved in sphingolipid
biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
903 |
| ORF length (spliced) |
840 |
| Entry clone length |
903 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
321T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1851.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTTTTATTAAGAGCAC |
| Rev primer name |
SPAC1851.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTGTTTTTTTACCTTTCCA |
| Amino acid length |
279 |
| Molecular weight |
31.3 |
| Isoelectric point (calc.) |
9.9 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
aggregates |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |