Gene name |
SPCC1235.04c |
Gene ID |
12/C09 |
Gene synonyms/obsolete |
|
Gene product |
FAD synthetase |
Entry clone |
Cloned |
ORF length (unspliced) |
899 |
ORF length (spliced) |
798 |
Entry clone length |
899 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
132T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1235.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGAGTTGGAACGTGT |
Rev primer name |
SPCC1235.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGATACTTCGGAAGACGCA |
Amino acid length |
265 |
Molecular weight |
30.6 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVGLQNRLSI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |