Gene name |
SPAC823.09c |
Gene ID |
12/C06 |
Gene synonyms/obsolete |
|
Gene product |
similar to
L-asparaginase; involved in glycoprotein degradation; no
apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
898 |
ORF length (spliced) |
858 |
Entry clone length |
898 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
435A:T / 650A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC823.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAATGGCTTGGTAAT |
Rev primer name |
SPAC823.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAACAAGTACGGGATACGA |
Amino acid length |
285 |
Molecular weight |
30.2 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |