Gene name |
SPCC1450.04 |
Gene ID |
12/B07 |
Gene synonyms/obsolete |
tef5 |
Gene product |
experimentally
characterised; elongation factor 1 (beta subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
892 |
ORF length (spliced) |
645 |
Entry clone length |
892 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
88A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1450.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTTTTTCTGATCTCAC |
Rev primer name |
SPCC1450.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAACTTGGACATAGCGGCG |
Amino acid length |
214 |
Molecular weight |
23.4 |
Isoelectric point (calc.) |
4.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |