| Gene name |
SPAC3G9.10c |
| Gene ID |
12/A06 |
| Gene synonyms/obsolete |
|
| Gene product |
exosome (RNase
complex); involved in rRNA processing; involved in mRNA
processing; involved in mRNA cleavage; possibly confers
antiviral activity by protecting cells from double-stranded
RNA (dsRNA) viruses; ribonuclease PH-like |
| Entry clone |
Cloned |
| ORF length (unspliced) |
888 |
| ORF length (spliced) |
729 |
| Entry clone length |
888 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3G9.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGAGATAGAATTTA |
| Rev primer name |
SPAC3G9.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCTGAGACTTGCAAGTGCC |
| Amino acid length |
242 |
| Molecular weight |
26.8 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB;
nucleus>cytosol; nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |