Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC5H10.08c
Gene ID 11/B08
Gene synonyms/obsolete
Gene product pantoate--beta-alanine ligase; involved in pantothenate biosynthesis (last step); possibly coexpressed with SPAC5H10.09c which is also involved in pantothenate biosynthesis
Entry clone Cloned
ORF length (unspliced) 852
ORF length (spliced)
Entry clone length 852
No. of intron 0
Sequence status Finished
Sequence results 7G:A / 175C:T / 372A:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC5H10.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGGTTCTTAAAGAGAA
Rev primer name SPAC5H10.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTGATGGTTGTTTGGATA
Amino acid length 283
Molecular weight 31.8
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus or cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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