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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17C9.14
Gene ID 11/A05
Gene synonyms/obsolete
Gene product involved in peroxisome biogenesis; farnesylated protein
Entry clone Cloned
ORF length (unspliced) 841
ORF length (spliced) 699
Entry clone length 841
No. of intron 1
Sequence status Finished
Sequence results 251T:C / 480A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17C9.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAAATCCAACTATTGA
Rev primer name SPAC17C9.14.Rv
Rev primer SEQ AGAAAGCTGGGTACTGGGTTGGACATCCTGCA
Amino acid length 232
Molecular weight 26.1
Isoelectric point (calc.) 4
Signal SEQ
No. of transmembrane domain 13
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: nucleus>cytosol
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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