Gene name |
SPAC17C9.14 |
Gene ID |
11/A05 |
Gene synonyms/obsolete |
|
Gene product |
involved in peroxisome
biogenesis; farnesylated protein |
Entry clone |
Cloned |
ORF length (unspliced) |
841 |
ORF length (spliced) |
699 |
Entry clone length |
841 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
251T:C / 480A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17C9.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAATCCAACTATTGA |
Rev primer name |
SPAC17C9.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTGGGTTGGACATCCTGCA |
Amino acid length |
232 |
Molecular weight |
26.1 |
Isoelectric point (calc.) |
4 |
Signal SEQ |
|
No. of transmembrane domain |
13 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
Leica |