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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC777.12c
Gene ID 10/H11
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned#
ORF length (unspliced) 837
ORF length (spliced) 717
Entry clone length 837
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC777.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAATTAATTTGTTTTT
Rev primer name SPCC777.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCTTTTTTGCAGCGGCG
Amino acid length 238
Molecular weight 26.8
Isoelectric point (calc.) 8.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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