| Gene name |
SPAC31G5.16c |
| Gene ID |
10/H03 |
| Gene synonyms/obsolete |
dpm1 |
| Gene product |
dolichol-phosphate
mannosyltransferase; involved in GPI anchor biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
836 |
| ORF length (spliced) |
711 |
| Entry clone length |
836 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC31G5.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAAATATAGCGTTTT |
| Rev primer name |
SPAC31G5.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGAATAGCAAGGAGAAG |
| Amino acid length |
236 |
| Molecular weight |
26.6 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKGVFSLLFI |
| Localization (YFP) |
ER; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |