Gene name |
SPBC3H7.11 |
Gene ID |
10/E08 |
Gene synonyms/obsolete |
|
Gene product |
methyltransferase; Sc
ABP140 is actin-binding protein; similar to Sp
SPBC21C3.07C |
Entry clone |
Cloned |
ORF length (unspliced) |
819 |
ORF length (spliced) |
747 |
Entry clone length |
819 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3H7.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGGTATATCAAAATT |
Rev primer name |
SPBC3H7.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAGCTTTTTCCAAACTCCT |
Amino acid length |
248 |
Molecular weight |
28.8 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLEVQPNLKI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|