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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC56F8.11
Gene ID 10/E04
Gene synonyms/obsolete
Gene product signal peptidase activity; signal peptidase complex
Entry clone Cloned
ORF length (unspliced) 817
ORF length (spliced) 558
Entry clone length 817
No. of intron 2
Sequence status Finished
Sequence results 59T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC56F8.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATCGTGGATACATTTAC
Rev primer name SPAC56F8.11.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCGGCTGTGTTATAATC
Amino acid length 185
Molecular weight 21.6
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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