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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1527.02
Gene ID 10/D12
Gene synonyms/obsolete sft2
Gene product involved in intracellular protein transport; involved in ER to Golgi transport
Entry clone Cloned
ORF length (unspliced) 815
ORF length (spliced) 606
Entry clone length 815
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1527.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGGAGTACGGTCTGA
Rev primer name SPAC1527.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGGGCAACCAGTTCGAAAGA
Amino acid length 201
Molecular weight 22.6
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTTMERLPI/LSIVFGVLHI
Localization (YFP) Golgi; vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 2 images) See all images

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