Gene name |
SPBC409.15 |
Gene ID |
10/B05 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
801 |
ORF length (spliced) |
540 |
Entry clone length |
801 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
150A:G / 205A:G /
565T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC409.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGACGACCCATCTAA |
Rev primer name |
SPBC409.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGACGTCTTTTCTGGACA |
Amino acid length |
179 |
Molecular weight |
20.5 |
Isoelectric point (calc.) |
3.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|