| Gene name |
SPAC26H5.13c |
| Gene ID |
10/B01 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved fungal
protein; sphingoid long chain base (LCB) kinase; similar to
S. cerevisiae YOR171C and YLR260W; 4 predicted
transmembrane helices |
| Entry clone |
Cloned |
| ORF length (unspliced) |
800 |
| ORF length (spliced) |
711 |
| Entry clone length |
800 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC26H5.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATTGTTTCGGCGTCC |
| Rev primer name |
SPAC26H5.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACACGACTTCATCCATGAGT |
| Amino acid length |
236 |
| Molecular weight |
27.1 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
4 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMYVSSVLML |
| Localization (YFP) |
Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |