Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC663.05c
Gene ID 10/A01
Gene synonyms/obsolete
Gene product histone chaperone; replication-coupling assembly factor (RCAF) component; anti-silencing protein
Entry clone Cloned
ORF length (unspliced) 789
ORF length (spliced)
Entry clone length 789
No. of intron 0
Sequence status Finished
Sequence results 375A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC663.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAATCGTGAATATCCT
Rev primer name SPCC663.05.Rv
Rev primer SEQ AGAAAGCTGGGTACTGAGACGTCTCTGGTTTC
Amino acid length 262
Molecular weight 29.4
Isoelectric point (calc.) 3.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.