| Gene name |
SPAC25G10.02 |
| Gene ID |
09/F08 |
| Gene synonyms/obsolete |
ydc2 |
| Gene product |
cruciform cutting
endonuclease; holliday junction resolvase component; SAP
domain; DNA-binding protein; SAP domain (SMART); involved in
mitochondrial DNA maintenance |
| Entry clone |
Cloned |
| ORF length (unspliced) |
777 |
| ORF length (spliced) |
|
| Entry clone length |
777 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC25G10.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTACTGTGAAACTTAG |
| Rev primer name |
SPAC25G10.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGTTTCAGAAACTGTTTG |
| Amino acid length |
258 |
| Molecular weight |
30.2 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery; cytoplasmic
dots |
| Comments for localization |
cytoplasmic dots
especially around vacuole |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |