Gene name |
SPAC10F6.06 |
Gene ID |
09/E11 |
Gene synonyms/obsolete |
vip1 |
Gene product |
rrm RNA recognition
motif; RNA-binding protein; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
774 |
ORF length (spliced) |
|
Entry clone length |
774 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC10F6.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCAACCAAGTTATTGT |
Rev primer name |
SPAC10F6.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCAGTGGTCTTTGACTCA |
Amino acid length |
257 |
Molecular weight |
27.4 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
large aggregates |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |