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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16E8.02
Gene ID 09/C04
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 754
ORF length (spliced) 669
Entry clone length 754
No. of intron 2
Sequence status Finished
Sequence results (-6)T:addition / 523T:C
Comments 5' terminus is frameshifted.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC16E8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTATGTAAGAATCTA
Rev primer name SPAC16E8.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAAACACAATACCATCGTCT
Amino acid length 222
Molecular weight 24.9
Isoelectric point (calc.) 7.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLLLTALVL/LLDNLIQSLFI/LSYCIRPLRL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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