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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC126.10
Gene ID 09/A02
Gene synonyms/obsolete iah1
Gene product isoamyl acetate hydrolytic enzyme
Entry clone Cloned
ORF length (unspliced) 741
ORF length (spliced)
Entry clone length 741
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC126.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGACTCTCAAGATAA
Rev primer name SPCC126.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTAAAGAGAATTTCAGAC
Amino acid length 246
Molecular weight 27.9
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization aggregates by over expression; bright moving dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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