Gene name |
SPBC23G7.03 |
Gene ID |
08/C04 |
Gene synonyms/obsolete |
SPBC18E5.13 |
Gene product |
translation initiation
factor 3; low similarity to bacterial translation initiation
factors; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
702 |
ORF length (spliced) |
|
Entry clone length |
702 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC23G7.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAGTTACTTACAGTT |
Rev primer name |
SPBC23G7.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAACTCAGAACTTTTAGAC |
Amino acid length |
233 |
Molecular weight |
27 |
Isoelectric point (calc.) |
10.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|