Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC965.07c
Gene ID 08/B03
Gene synonyms/obsolete gst2
Gene product glutathione S-transferase; involved in oxidative stress response
Entry clone Cloned
ORF length (unspliced) 693
ORF length (spliced)
Entry clone length 693
No. of intron 0
Sequence status Finished
Sequence results 15T:G / 120A:G / 520T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC965.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTCATTTTACTTTATA
Rev primer name SPCC965.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATGTTGTTCTTTGGCCTTT
Amino acid length 230
Molecular weight 26.8
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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