Gene name |
SPAC17A2.10c |
Gene ID |
08/A10 |
Gene synonyms/obsolete |
|
Gene product |
very hypothetical
protein |
Entry clone |
Cloned in 2004
trial |
ORF length (unspliced) |
693 |
ORF length (spliced) |
|
Entry clone length |
693 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC17A2.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGTGCGTTAATGTTTG |
Rev primer name |
SPAC17A2.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACAAACACACGGCAAAGAA |
Amino acid length |
230 |
Molecular weight |
26.5 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLFRCLLL/LFSLSHLLSL/LPRLFCLCL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |