Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17H9.03c
Gene ID 08/A08
Gene synonyms/obsolete
Gene product predicted coiled-coil region; possibly involved in DNA repair
Entry clone Cloned
ORF length (unspliced) 693
ORF length (spliced)
Entry clone length 693
No. of intron 0
Sequence status Finished
Sequence results 203A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17H9.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATCAGGTTACTCAATT
Rev primer name SPAC17H9.03.Rv
Rev primer SEQ AGAAAGCTGGGTATGATGGACTGTTGAGCGTA
Amino acid length 230
Molecular weight 26
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVYIKQLHL
Localization (YFP) cytosol=nucleus; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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