| Gene name |
SPAC644.13c |
| Gene ID |
07/G10 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in targeting
and fusion of ER to Golgi transport vesicles; not Sc YIP1
homolog; no apparent Sc ortholog; YIP1 family; GTPase
interacting protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
678 |
| ORF length (spliced) |
|
| Entry clone length |
678 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
17A:T / 656T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC644.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAGACATAGGAAAACA |
| Rev primer name |
SPAC644.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAAAAGATAATCCAAGCT |
| Amino acid length |
225 |
| Molecular weight |
25.3 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWGPLIFSLVI/LLAVYPLFL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |