| Gene name |
SPBC16E9.12c |
| Gene ID |
07/F06 |
| Gene synonyms/obsolete |
pab2 |
| Gene product |
poly(A)-binding
protein; involved in polyadenylation; RRM RNA recognition
motif |
| Entry clone |
Cloned |
| ORF length (unspliced) |
663 |
| ORF length (spliced) |
501 |
| Entry clone length |
663 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
364A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16E9.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGATCAAGATGCCTT |
| Rev primer name |
SPBC16E9.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATACGGAGCGAAACCACGG |
| Amino acid length |
166 |
| Molecular weight |
18.4 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
142/144 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus; nuclear
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss, DeltaVision
|