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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC330.12c
Gene ID 05/E06
Gene synonyms/obsolete sdh3
Gene product putative succinate dehydrogenase cytochrome
Entry clone Cloned
ORF length (unspliced) 543
ORF length (spliced)
Entry clone length 543
No. of intron 0
Sequence status Finished
Sequence results 465T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC330.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTCGCAACTCGCTCATT
Rev primer name SPCC330.12.Rv
Rev primer SEQ AGAAAGCTGGGTAAATCATCGCAAAATAGCCA
Amino acid length 180
Molecular weight 20.1
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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