Gene name |
SPAPJ760.03c |
Gene ID |
04/H04 |
Gene synonyms/obsolete |
|
Gene product |
Sp specific families;
hypothetical protein; GPI anchored protein; glycoprotein; no
apparent Sc ortholog; threonine-rich protein |
Entry clone |
Cloned |
ORF length (unspliced) |
501 |
ORF length (spliced) |
|
Entry clone length |
501 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
192A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPJ760.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTCTTCGCTCTATCTT |
Rev primer name |
SPAPJ760.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCAACAAAGGAGACACTG |
Amino acid length |
166 |
Molecular weight |
17.3 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |