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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2E1P5.02c
Gene ID 04/F11
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 492
ORF length (spliced)
Entry clone length 492
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2E1P5.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGAAGTTAGAAAATAA
Rev primer name SPAC2E1P5.02.Rv
Rev primer SEQ AGAAAGCTGGGTACAATATACCCGCTGGATTC
Amino acid length 163
Molecular weight 18.6
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery, especially at site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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