| Gene name |
SPAC1556.05c |
| Gene ID |
04/C10 |
| Gene synonyms/obsolete |
|
| Gene product |
CGR1 family; involved
in rRNA processing; conserved fungal protein; involved in
ribosome biogenesis and assembly; predicted coiled-coil
region |
| Entry clone |
Cloned |
| ORF length (unspliced) |
470 |
| ORF length (spliced) |
336 |
| Entry clone length |
470 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1556.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTAATGGAACTAAGGG |
| Rev primer name |
SPAC1556.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCGCTCTTTCAACATTTTG |
| Amino acid length |
111 |
| Molecular weight |
13.5 |
| Isoelectric point (calc.) |
11.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
95/97 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |